The laser module has 3 single photon lasers (Ar, HeNe 543, HeNe 633) which allow excitation lines of 458, 488, 514, 543, 568, and 633 nm. The detector module has 3 confocal detectors (photomultiplier tubes with pinholes). The scan module can be fitted on either the Axiovert 200M or the Axioplan 2, both fully motorised microsopes controlled via a standard Pentium PC. The Axioplan motorised research microscope is equipped with 3 Plan Neofluar objectives; 10X, 20X, 50X The Axiovert 100M SP motorised research microscope is equipped with 3 Plan Neofluar objectives; 10X, 20 X, 40X (1.3NA oil) and a Plan Apochromatic objective; 63X (1.4 NA oil) All objective lenses are ICS lenses (infinity colour corrected system) Both microscopes are configured for transmitted light, brightfield, phase contrast, fluorescence (filter sets for UV, blue & green excitation), reflected light, DIC (differential interference contrast).
The Zeiss LSM software, which runs on Windows NT 4.0 Operating System, provides a wide range of image processing functions,
including all standard 2D/3D functions (stereo,projection, depth colour coding,viewing from any angle), digital processing of voxels
and 3D measurement functions and parametres.
Additional Physiology/ time series software, Topography software and Macro recorder/ editor software are also available.
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Additional Features
- Acousto Optical Tuneable Filter (AOFT) delivering exact control over laser lines and intensities with beam on and off with pin point accuracy
- MMS-VIS Spectrometer, wavelenght range 320-950nm, Hamamatsu photodiode array detector
Imaging Modes:
- single optical sections, xy scans
- time lapse imaging, xyt scans
- z series and 3D imaging, xyz scans
- 4D imaging, xyzt scans
- X-Z imaging, xz scans
- reflected light imaging
- transmitted light imaging
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