Confocal microscopy offers several advantages over conventional widefield optical microscopy, including the ability to control depth of field, elimination or reduction of background information away from the focal plane (that leads to image degradation), and the capability to collect serial optical sections from thick specimens. The basic key to the confocal approach is the use of spatial filtering techniques to eliminate out-of-focus light or glare in specimens whose thickness exceeds the immediate plane of focus.
LSM 510 META
The LSM 510 META scanning system can be mounted on either a motorized inverted stand (Zeiss Axiovert 200M) or a manual upright stand (Zeiss Axioplan 2). The system offers 6 excitation wavelengths (458nm, 477nm, 488nm, 514nm, 543nm and 633nm) and for detection, three separate reflected light PMTs, each with its own adjustable pinhole and emission filter wheel.
Z sectioning and 3D reconstruction
Time series for following cellular events (e.g., physiology experiments)
Co-localization of multiple proteins and/or dyes
Emission fingerprinting (lambda stacks scan)
FRAP (Fluorescence Recovery after Photobleaching) experiments
Topography: visualization of 3D surfaces (e.g., materials analysis), plus measurement functions (roughness, waviness, surface areas, volumes).